Specific Separation of Cells on Affinity Columns
نویسندگان
چکیده
منابع مشابه
Cell separation on affinity columns: the isolation of immunospecific precursor cells from unimmunized mice (lactoside hapten-lymphocyte receptors-immunology).
We have used affinity columns to isolate from the spleens of unimmunized mice a population of lymphocytes that specifically bind a lactoside hapten. These cells are able to initiate an antibody response to azophenyl-beta-lactoside when transferred to appropriate irradiated recipients.
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Glass and plastic bead columns coated with antigenic protein molecules were used as an immunological filter for cell populations containing immune cells of relevant specificity. A selective elimination of these immune cells from the passing cell suspension was regularly noted and it approached, in some experiments, complete abolition of the specific immune reactivity of the filtered cell popula...
متن کاملSome general methods of preparing affinity columns.
Some general methods of covalent coupling of nucleotides, especially derivatized nucleotides, polynucleotides and cofactors to insoluble polymers are described in this paper. Wherever necessary individual methods also carry some information on the binding of enzymes to the same polymers to serve as a guide to the efficiency of the coupling methods.
متن کاملSeparation of nucleic acid degradation products on partition columns.
Since the discovery that 5-methylcytosine is a minor constituent of deoxyribonucleic acid (1, 2) several other minor components have been found in nucleic acids. In particular, ribonucleic acid isolated from bacterial, plant, and animal sources contains trace amounts of a series of methylated purines (3-5), pyrimidine derivatives (4, 6, 7), and 2 (or 3)-O-methylribose (8). It has been reported ...
متن کاملThe Effect of Maldistribution on Separation in Packed Distillation Columns
For a packed bed in a distillation column, fmax is the maximum fractional liquid maldistribution that can be tolerated in a parallel column model whilst still being able to achieve the design separation. It was previously shown that fmax can be easily calculated from a conventional column simulation output and it is a measure of the sensitivity of a packed bed to maldistribution. In this paper,...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1970
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.66.3.685